Fottea 2022, 22(1):122-136 | DOI: 10.5507/fot.2021.019

Rhodomonas storeatuloformis sp. nov. (Cryptophyceae, Pyrenomonadaceae), a new cryptomonad from the Black Sea: morphology versus molecular phylogeny

Antonina N. Khanaychenko1, Olga V. Popova2, Olga A. Rylkova1, Vladimir V. Aleoshin2, 3, Larisa O. Aganesova1, Maria Saburova4*
1 A.O. Kovalevsky Institute of Biology of the Southern Seas, Russian Academy of Sciences, Leninskii ave., 38/3, Moscow, 119991, Russian Federation
2 Belozersky Institute for Physico-Chemical Biology, Lomonosov Moscow State University, Leninskye Gory, 1/40, 119991 Moscow, Russian Federation
3 Kharkevich Institute for Information Transmission Problems, Russian Academy of Sciences, Bolshoy Karetny per., 19/1, 127051 Moscow, Russian Federation
4 Environment and Life Sciences Research Center, Kuwait Institute for Scientific Research, P.O. BOX 1638, Salmiya 22017, Kuwait; *Corresponding author e-mail: msaburova@gmail.com

We established a new cryptomonad species, Rhodomonas storeatuloformis sp. nov., based on morphological and molecular characters of cultured strain isolated from the Black Sea. Cells are slightly dorsoventrally flattened, obloid in shape, 12-19 µm in length and 5-10 µm in width, with light-brown, parietal H-shaped chloroplast and a central pyrenoid. Two unequal flagella are located subapically in a short V-shaped vestibulum with a ligule on the left side. Cells are covered by a sheet-like papillate perisplast with numerous small underlain ejectosomes; discharged ejectosomes form distinctly visible minute pores. Mid-ventral band is absent. In general, cell morphology is very similar to described strains of Storeatula genus. However, phylogenetic analyses based on sequences of the partial nuclear 18S, 28S rDNA, and complete internal transcribed spacer (ITS) region of rDNA placed the novel cryptomonad strain within Rhodomonas genus as a separate clade. The predicted secondary structure of nuclear rDNA ITS2 has numerous compensatory base changes compared to the closest relative strains that support the distinction of the novel species among Rhodomonas taxa. The present results contribute to the study on still hidden cryptomonad biodiversity in the Black Sea. Contradiction between morphology and phylogenetic data in R. storeatuloformis further argues for revision of the generic delineations in the family Pyrenomonadaceae.

Keywords: Black Sea, compensatory base changes, ITS2; rDNA phylogeny, Rhodomonas storeatuloformis sp. nov., SEM, taxonomy

Received: May 25, 2021; Revised: August 3, 2021; Accepted: September 29, 2021; Prepublished online: January 11, 2022; Published: April 1, 2022  Show citation

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Khanaychenko, A.N., Popova, O.V., Rylkova, O.A., Aleoshin, V.V., Aganesova, L.O., & Saburova, M. (2022). Rhodomonas storeatuloformis sp. nov. (Cryptophyceae, Pyrenomonadaceae), a new cryptomonad from the Black Sea: morphology versus molecular phylogeny. Fottea, 22(1), 122-136. doi: 10.5507/fot.2021.019
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